Journal: International journal of oncology

Article Title: Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer.

doi: 10.3892/ijo.2011.1247

Figure Lengend Snippet: Figure 2. (a), Scattergram of serum CXCL10 in stage I-IV colorectal cancer patients and control subjects (p<0.0001). (b), Scattergram of serum CXCL10 subdivided by pathological T stage (p=0.0101). (c), Scattergram of serum CXCL10 subdivided by clinical H stage (p=0.0004). (d), The relationships between serum CXCL10 and serum CEA level (r=0.2853, p<0.0001).

Article Snippet: Primary anti-CXCL10 goat polyclonal antibody (clone AF976, R&D Systems, MN, USA) and anti-CXCR3 rabbit polyclonal antibody (clone ab8023, Abcam, Cambridge, UK) at a dilution of 1:25 were used for the labeled streptavidinbiotin method (LASB2 kit/HRP, Dako Cytomation).

Techniques: Control

Journal: International journal of oncology

Article Title: Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer.

doi: 10.3892/ijo.2011.1247

Figure Lengend Snippet: Figure 3. (a), Cancer specific survival curves, for 218 patients with colorectal cancer, according to serum CXCL10 level. Patients with higher serum CXCL10 had significantly poorer prognoses than those with lower serum CXCL10 levels (p<0.0001, log-rank test); cut-off value = 199 pg/ml. (b), Cancer specific survival curves for 163 patients in stage I-III with curative intent, according to serum CXCL10 level. Patients with higher serum CXCL10 also had significantly poorer prognoses than those with lower serum CXCL10 levels (p=0.0372, log-rank test); cut-off value = 153 pg/ml.

Article Snippet: Primary anti-CXCL10 goat polyclonal antibody (clone AF976, R&D Systems, MN, USA) and anti-CXCR3 rabbit polyclonal antibody (clone ab8023, Abcam, Cambridge, UK) at a dilution of 1:25 were used for the labeled streptavidinbiotin method (LASB2 kit/HRP, Dako Cytomation).

Techniques:

Journal: International journal of oncology

Article Title: Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer.

doi: 10.3892/ijo.2011.1247

Figure Lengend Snippet: Figure 4. CXCL10 protein expression was assessed using immunohistochemistry in normal mucosa with primary CRC (a), primary colorectal cancer (b) and liver metastasis tissue (c). Magnification, x40. Scale bar = 500 µm.

Article Snippet: Primary anti-CXCL10 goat polyclonal antibody (clone AF976, R&D Systems, MN, USA) and anti-CXCR3 rabbit polyclonal antibody (clone ab8023, Abcam, Cambridge, UK) at a dilution of 1:25 were used for the labeled streptavidinbiotin method (LASB2 kit/HRP, Dako Cytomation).

Techniques: Expressing, Immunohistochemistry

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 1. VCAM-1, P-selectin, sTNFR-1, and CXCL16 are elevated in the urine in spontaneous lupus nephritis in three different mouse models of lupus. The levels of the above molecules were determined by ELISA in 24-h urine samples obtained from 6-mo-old B6.Sle1.lpr, MRL.lpr, and NZM2410 lupus mice (all of which exhibited proteinuria and glomerulonephritis), as well as 6-mo-old B6 controls (A). Indicated p values per- tain to Student’s t test or Mann-Whitney U test compar- isons of B6 urine vs the other groups (, p 0.05; , p 0.01; , p 0.001). n 5 mice/group. For each urine sample depicted in Fig. 1A, the 24-h urine level of total protein was plotted against the 24-h urine levels of the four molecules (B). Typically, healthy B6 mice do not excrete 1 mg of protein over a 24-h period (18, 19).

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 2. Urinary VCAM-1, P-selec- tin, sTNFR-1, and CXCL16 are higher than the corresponding serum levels. Se- rum and urine levels of the above four molecules were determined by ELISA, us- ing samples from 2-mo-old B6, 2-mo-old MRL.lpr, and 6-mo-old MRL.lpr mice. In- dicated p values pertain to Student’s t test or Mann-Whitney U test comparisons of B6 urine vs the other groups (indicated be- low the x-axis), or young vs old MRL.lpr samples, as indicated above each plot (, p 0.05; , p 0.01; , p 0.001). n 12 mice/group.

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 3. The urinary enrichment of VCAM-1 and CXCL16 is also noted in other strains with spontaneous lupus nephritis. Serum and urine levels of VCAM-1 and CXCL16 were determined by ELISA, using samples from 2- to 5-mo-old B6, B6.Sle1.lpr, and BXSB mice, as in- dicated. Displayed p values pertain to the Student t test or Mann-Whit- ney U test comparisons of young vs old mice (, p 0.05; , p 0.01; , p 0.001); n 6 mice/group.

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 4. VCAM-1, TNFR-1, P-selectin, and CXCL16 are hyperex- pressed within the diseased kidneys in lupus. Renal cortical lysates were prepared from B6 control, as well as BXSB and MRL.lpr mice, aged 5–6 mo. The levels of the four molecules were determined by ELISA. Indicated p values pertain to Student’s t test or Mann-Whitney U test comparisons of B6 vs other strains (, p 0.05; , p 0.01; , p 0.001). n 6 mice/group. Group means are indicated by the horizontal bars.

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: Control, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 5. CXCL16, p-selectin, sTNFR-1, and VCAM-1 may be ex- pressed in the kidneys. Immunostaining of mouse kidneys from 2- and 7-mo-old MRL.lpr mice was performed using Abs against the above four molecules. Shown figures are representative of the histology seen in five mice per group.

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: Immunostaining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 6. Urine VCAM-1, P-selectin, sTNFR-1, and CXCL16 are also elevated in human lupus nephritis. A, Spot urine samples from 38 SLE patients (Table I), 15 healthy adults, and 6 RA patients were assayed for the levels of VCAM-1, P-selectin, sTNFR-1, and CXCL16. Indicated p values pertain to Student’s t test or Mann-Whitney U test comparisons of the urinary levels of each molecule in patients against that in normal controls (indicated below the x-axis), or SLE vs RA, as indicated at the top (, p 0.05; , p 0.01; , p 0.001). Shown also are the correlation profiles of these urinary molecules (normalized to urine creatinine) against the corresponding urine protein-creatinine ratios (B) and SLEDAI scores (C). R: correlation coefficient.

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: MANN-WHITNEY

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 7. Specificity/sensitivity ROC curves per- taining to the use of urinary VCAM-1 (A), CXCL16 (B), sTNFR-1 (C), and P-selectin (D) for discriminating SLE patients from normal controls. The data shown in Fig. 6 were used to generate the shown ROC curves, which plot the specificity and sensitivity profiles of the indicated uri- nary molecules in distinguishing SLE patients from nor- mal controls. Indicated are the corresponding AUC values.

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 8. SLE patients with active ne- phritis exhibit the highest levels of urine VCAM-1, P-selectin, sTNFR-1, and CXCL16. Urine levels of the indicated molecules were assayed in SLE patients and normal controls. The SLE patients were divided into four groups: (1) inactive SLE (SLEDAI 0); (2) mild-active, non- renal SLE (SLEDAI 1–5, renal SLE- DAI 0); (3) severe-active, nonrenal SLE (SLEDAI 6; renal SLEDAI 0); (4) ac- tive renal SLE (renal SLEDAI 0). Indi- cated p values pertain to Student’s t test or Mann-Whitney U test comparisons of each subject group vs “mild-active, nonrenal SLE” (, p 0.05; , p 0.01; , p 0.001).

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: MANN-WHITNEY

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 9. Specificity/sensitivity ROC curves per- taining to the use of urinary VCAM-1 (A) and CXCL16 (B) for discriminating SLE patients with active renal dis- ease. The data shown in Fig. 8 were used to generate the shown ROC curves, which plot the specificity and sensi- tivity profiles of the indicated urinary molecules in dis- tinguishing SLE patients with active renal disease from all other SLE patients. Indicated are the corresponding AUC values.

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

doi: 10.4049/jimmunol.179.10.7166

Figure Lengend Snippet: FIGURE 10. Urine vs serum levels of VCAM-1, P-selectin, sTNFR-1, and CXCL16 in human lupus. Serum levels of the above four molecules were also assayed in SLE patients with inactive SLE (SLEDAI 0; n 5, randomly selected from the patients listed in Ta- ble I) and in patients with “active SLE” (SLEDAI 12; n 12, randomly se- lected from the patients listed in Table I). Displayed are the absolute levels (left column) as well as the albumin- normalized values (middle column) for the four molecules assayed. Displayed in the right column are the urine-serum ratios of these four molecules, after normalization against the correspond- ing albumin levels. Indicated p values pertain to Student’s t test or Mann- Whitney U test comparisons of patients vs “normal” (indicated below the x-axis), or “inactive” vs “active” pa- tients, as indicated above each plot (, p 0.05; , p 0.01; , p 0.001).

Article Snippet: Kidney sections obtained from 2- or 6-mo-old mice were stained with the following primary Abs: goat anti-mouse CXCL16 Ab (no. AF503; R&D Systems), goat anti-mouse TNFR-1 Ab (no. BAF425; R&D Systems), goat anti-mouse VCAM-1 (no. BAF643; R&D Systems) Ab, or goat anti-mouse P-selectin Ab (no. SC-6941; Santa Cruz Biotechnology) or the respective isotype control Abs, and developed as described previously (21).

Techniques: MANN-WHITNEY

Journal: Nature communications

Article Title: Targeting IL-17A enhances imatinib efficacy in Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia.

doi: 10.1038/s41467-023-44270-3

Figure Lengend Snippet: Fig. 5 | IL-17A stimulates the secretion of CXCL16 from leukemia cells, pro- moting the differentiation and migration of Th17 cells. a B220+ cells and Th17 cells in the BM niche in WT mice and BCR-ABLtTA mice (n = 3 mice per group) were subjected to immunofluorescence staining. Representative images were shown. b The numbers of Th17 cells migrating to culture medium or to leukemia cells treated with or without 50 ng/ml rhIL-17A were determined by cell counting and FCS analysis. c Real-time PCR analysis of the relative mRNA levels of chemokine genes in SupB15 (left) and BV173 cells (right) treated with rhIL-17A (100 ng/ml) or PBS control. d The human IL-5, CXCL5, CCL5 and CXCL16 levels in the culture supernatant of SupB15 cells treated with or without rhIL-17A (100 ng/ml) were measured by ELISA. e The CXCL16 concentrations in the serum of BCR-ABLtTA mice and WT mice (n = 5 mice per group) were quantified with a Mouse Chemokine Assay Q1 (Raybiotech). f The CXCL16 concentrations in the serum of HDs (n = 7 samples)

Article Snippet: For the coculture assay, viable Th17 cells were cocultured with B-ALL cells at a ratio of 1:1, and recombinant human CXCL16 (PeproTech, 300-55), recombinant human IL-17A (PeproTech, 200-17), the antihuman IL-17A neutralizing antibody (Bio X Cell, SIM0013), the antihuman CXCL16 neutralizing antibody (R&D, AF976), anti-goat IgG (R&D, AB-108-C) or recombinant human IgG1 Fc (Bio X Cell, BE0096) was added to the indicated coculture systems.

Techniques: Migration, Staining, Cell Counting, Real-time Polymerase Chain Reaction, Control, Enzyme-linked Immunosorbent Assay